¤½¤ì¤¾¤ì¤Î¥¨¥ó¥È¥ê¤Î¤Ë¤Ï¥·¥ê¥¢¥ëÈֹ椬ÉÕ¤±¤é¤ì¤Æ¤¤¤Æ¡¢ÊѰۤμïÎà¤Ë¤è¤Ã¤Æ "natural"¤¢¤ë¤¤¤Ï"artificial"¤ÈµºÜ¤µ¤ì¤Æ¤¤¤Þ¤¹¡£ 'PURPOSE'¤Ï¤½¤ÎÏÀʸ¤ÎÌÜŪ¤òû¤¯¤Þ¤È¤á¤¿¤â¤Î¤Ç¤¹¡£ 'CROSS-REFERENCE'¤Ï¡¢¤½¤ÎÏÀʸ¤Ç°·¤Ã¤¿¥¿¥ó¥Ñ¥¯¼Á¤Î¥³¡¼¥É̾¤Ç¤¹¡£ ¸¶Â§¤È¤·¤ÆSwissprot¤Î¥³¡¼¥É̾¤¬µºÜ¤µ¤ì¡¢ ¾ì¹ç¤Ë¤è¤Ã¤Æ¤Ï¡¢PIR¡¢ genbank¡¢PDB¤Î¥³¡¼¥É¤¬µºÜ¤µ¤ì¤Æ¤¤¤Þ¤¹¡£ 'N-TERMINAL'¤Ï»Ä´ð¤ÎÈÖ¹æÉÕ¤±¤òÌÀ¼¨¤¹¤ë¤¿¤á¡¢ ¤½¤ÎÏÀʸ¤Ë¤ª¤¤¤Æ£±»Ä´ðÌܤȤµ¤ì¤¿Éô°Ì¤ÎºÇ½é¤Î£µ»Ä´ð¤ò¼¨¤·¤¿¤â¤Î¤Ç¤¹¡£ 'CHANGE'¤Ï¡¢ÊѰۤΰÌÃ֤ȼïÎà¤òµºÜ¤¹¤ë¹àÌܤǤ¹¡£»Ä´ðÃÖ´¹¡¢ÁÞÆþ¡¢ºï½ü ¤Ê¤ÉÍÍ¡¹¤ÊÊÑ°Û¤ËÂбþ¤·¤Þ¤¹¡£CHANGE¹àÌܤˤĤ¤¤Æ¤Ï¸å¤Ë¾Ü¤·¤¯ÀâÌÀ¤·¤Þ¤¹¡£ ³ÆCHANGE¹àÌܤϣ±¤Ä¤ÎÊÑ°Û¥¿¥ó¥Ñ¥¯¼Á¤ËÂбþ¤·¤Æ¤ª¤ê¡¢¥¨¥ó¥È¥ê¡¼Æâ¤Î CHANGE¹àÌܤοô¤Ï¡¢¤½¤ÎÏÀʸ¤Ç°·¤Ã¤¿ÊÑ°Û¥¿¥ó¥Ñ¥¯¼Á¤Î¿ô¤ËÁêÅö¤·¤Þ¤¹¡£ ÊÑ°Û¥¿¥ó¥Ñ¥¯¼Á¤Ë´Ñ»¡¤µ¤ì¤¿µ¡Ç½Åª¡¦¹½Â¤Åª¤ÊÆÃħ¤Ï¡¢'CHANGE'¤Î ¤¹¤°¤¢¤È¤ËµºÜ¤µ¤ì¤Þ¤¹¡£ÌîÀ¸·¿¤È¤ÎÁêÂÐŪ¤ÊÈæ³Ó¤Ë¤è¤ê¡¢ [- -], [-], [=], [+], [+ +]¤Î°õ¤¬ÉÕ¤¤¤Æ¤¤¤Þ¤¹¡£ ÊäÂŪ¤ÊÀâÌÀ¤Ï'COMMENT'¤Î¹àÌܤ˵ºÜ¤µ¤ì¤Þ¤¹¡£
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****** CONTENTS ******
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ENTRY *___++++ - ****** +++++++
| | | |
| | |_ Kind of mutations |_Article number
| | Artificial in PRF/LITDB
| | Natural
| | Variant
| | Chemical
| | Chimera
| | Enzymatic
| | Synthetic
| | Signal
| |
| |_ Number in "MUTANT DATABASE"
|
|_ A; article
R; review
B; preliminary entry, type B
C; preliminary entry, type C
PMD¤Î¥³¡¼¥É̾¤Ï 'ENTRY'¹à¤ÎÃæ¤Î"A890481", "C973436" ¤Ê¤É¡¢ "[ARBC] + [Year] + [Number]"¤Î·Á¤Çµ½Ò¤µ¤ì¤Æ¤¤¤Þ¤¹¡£ÀèƬ¤Î ¥¢¥ë¥Õ¥¡¥Ù¥Ã¥È¤ÏA,R,B,C¤Î£´¼ïÎढ¤ê¡¢¤½¤ì¤¾¤ì¼¡¤Î°ÕÌ£¤òɽ¤·¤Þ¤¹¡£
ÊѰۤˤϡ¢Ã±½ã¤ÊÅÀÊÑ°Û¤«¤é¡¢Ê£»¨¤Ê¥¥á¥éÂΤκîÀ®¤Þ¤Ç¡¢ÍÍ¡¹¤Ê ¼ïÎब¤¢¤ê¤Þ¤¹¡£°Ê²¼¤ËÎã¤ò¤¢¤²¤Æ¡¢PMD¤Ç¤ÎÊѰۤεºÜË¡¤ò¼¨¤·¤Þ¤¹¡£
CHANGE His 44 Arg
; His-44 ¤ò Arg¤ËÃÖ´¹¡£
CHANGE Gly-Asp 124-125 Asp-Val
; Gly-124 ¤È Asp-125 ¤ò¤½¤ì¤¾¤ì Asp¤È Val¤ËÃÖ´¹¡£
CHANGE Phe--Phe 23-25 Tyr-Phe-Tyr
; Phe-23 ¤«¤é Phe-25 ¤Þ¤Ç¤ÎÎΰè¤òTyr-Phe-Tyr¤ËÃÖ´¹¡£
CHANGE Lys 35 (deletion)
; Lys-35¤òºï½ü¡£
CHANGE Glu--Gln 32-46 (deletion)
; Glu-32¤«¤éGln-46¤Þ¤Ç¤ÎÎΰè¤òºï½ü¡£
CHANGE Ala--Arg 14-32 Gly (deletion)
; Ala-14 ¤«¤é Arg-32 ¤Þ¤Ç¤Î Îΰè¤ò Gly ¤ËÃÖ´¹¡£·ë²ÌŪ¤Êºï½ü¡£
CHANGE Pro 76 (termination)
; Pro 76¤ò½ª»ß¥³¥É¥ó¤ËÊѤ¨¡¢¤½¤ì°Ê¹ß¤òºï½ü¤¹¤ë¡£
CHANGE Phe^Gly 441^442 Phe (insertion)
; Phe-441 ¤È Gly-442 ¤Î´Ö¤Ë Phe¤òÁÞÆþ¤¹¤ë¡£
CHANGE ^Thr ^1 Val (extension)
; NËöü Thr-1 ¤Ë Val¤òÉղ乤롣
CHANGE Ala^ 53^ Tyr-Ser (extension)
; CËöü Ala-53 ¤Ë Tyr-Ser ¤òÉղ乤롣
CHANGE Ala--Val 24-27 20AAs fragment (insertion)
; Ala-24 ¤«¤é Val-27 ¤Þ¤Ç¤ÎÎΰè¤ò20»Ä´ð¤Î¥Õ¥é¥°¥á¥ó¥È¤ÇÃÖ´¹¡£·ë²ÌŪ¤ÊÁÞÆþ¡£
VARIATION Val/Ile 36
Glu/Gln 102
; 36ÈÖÌܤ¬Val¤«Ile¡¢102ÈÖÌܤ¬Glu¤«Gln¡£ÁȤ߹ç¤ï¤»¤ÏÌÀ¤é¤«¤Ç¤Ê¤¤¡£
VARIATION Tyr 35 His (eglin c, No., eglin b)
; eglin c ¤ÎTyr-35¤ò eglin b¤ÎHis¤ËÃÖ´¹
1998ǯ4·îÆþÎÏʬ¤«¤é¡¢¤è¤ê¤ï¤«¤ê¤ä¤¹¤¤¤è¤¦¤Ë°Ê²¼¤Î·Á¼°¤Îɽµ¤âºÎÍѤ·¤Æ¤¤¤Þ¤¹¡£
CHANGE-POINT His 44 Arg
; His-44 ¤ò Arg¤ËÃÖ´¹¡£
CHANGE-MODIFY Ala 63 (L-parafluorophenyl)Ala
; Ala-63 ¤ò (L-parafluorophenyl)Ala¤Ë½¤¾þ¡£
CHANGE-DELETE Lys 35
; Deletion of ºï½ü
CHANGE-DELETE Glu--Gln 32-46
; Glu-32¤«¤éGln-46¤òºï½ü
CHANGE-DELETE Leu 8
-DELETE Glu--Gln 15-26
; Leu-8¤È¡¢Glu-15¤«¤éGln-26¤òºï½ü
CHANGE-STOP Pro 76 (termination)
; Pro 76 ¤ò½ª»ß¥³¥É¥ó¤ËÊѤ¨¤ë¡£
CHANGE-INSERT Phe^Gly 441^442 GALKE
;Phe-441 ¤È Gly-442 ¤Î´Ö¤Ë Gly-Ala-Leu-Lys-Glu¤òÁÞÆþ¤¹¤ë¡£
CHANGE-EXTEND Ala^ 53^ YVACN
;CËöü Ala-53 ¤Ë Tyr-Val-Ala-Cys-Asn ¤òÉղ乤롣
CHANGE-REPLACE Ala--Arg 14-16 Gly-Thr-Phe-Val
;Ala-14¤é¤«Arg-16¤Þ¤Ç¤òGly-Thr-Phe-Val¤ÇÃÖ¤´¹¤¨¤ë¡£
CHANGE-REPLACE Val--Ile 64-70 GLIVPHALLSFRTHIIK
;Val-64¤«¤éIle-70¤Þ¤Ç¤ò¡¢GLIVPHALLSFRTHIIK¤ÇÃÖ¤´¹¤¨¤ë¡£
CHANGE-FRAGMENT Ala--Val 35-187 (fragmented)
; Ala--35 to Val-187¤Þ¤Ç¤Î¥Õ¥é¥°¥á¥ó¥È¤Ë¤¹¤ë¡£
CHANGE-FRAME Ala 39 (frameshift)
; Ala-38¤Î¸å¤Ë¡¢¥Õ¥ì¡¼¥à¥·¥Õ¥È¤òÆþ¤ì¤ë¡£
CHANGE-POINT His 3 Ala
-POINT Asp 11 Asn
-REPLACE Ala--Arg 14-16 GTFVAP
;ÅÀÊÑ°Û¤ÈÃÖ¤´¹¤¨¤ÎÁȤ߹ç¤ï¤»¡£ His-3¤òAla¤Ë¡¢Asp-11¤òAsn¤ËÊѤ¨¡¢
Ala-14¤«¤éArg-16¤òGly-Thr-Phe-Val-Ala-Pro¤ËÃÖ¤´¹¤¨¤ë¡£
CHANGE-REPLACE Ala--Arg 14-16 GTFVM
-INSERT Phe^Gly 441^442 GALKE
; ÁÞÆþ¤ÈÃÖ¤´¹¤¨¤ÎÁȤ߹ç¤ï¤»¡£Ala-1¤«¤é Arg-16 ¤ò Gly-Thr-Phe-Val-Met
¤ËÃÖ¤´¹¤¨¡¢Phe-441¤ÈGly-442¤Î´Ö¤ËGly-Ala-Leu-Lys-Glu¤òÁÞÆþ¤¹¤ë¡£
¥¥á¥é¤¿¤ó¤Ñ¤¯¼Á¤ÎÆþÎÏÎã
Îã1
CROSS-REFERENCE O4RBP2
PROTEIN Cytochrome P450 2C2
SOURCE Rabbit
N-TERMINAL MDLVV
CROSS-REFERENCE O4RBP1
PROTEIN Cytochrome P450 2C1
SOURCE Rabbit
N-TERMINAL MDPVV
EXPRESSION-SYSTEM COS-1 cells
CHANGE-CHIMERA Arg--Val 307-490 of cytochrome P450 2C2 is replaced by the
corresponding region of cytochrome P450 2C1.
-SEQUENCE MDLVVVLGLCLSCLLLLSLWKQSHGGGKLPPGPTPFPILGNVLQLDFKDLSKSLTNLSKV
YGPVFTVYLGMKPTVVVHGYEAVKEALVDLGHELSGRSRFLVTAKLNKGFGVIFSNGKRW
TETRRFSLMTLRNFGMGKRSIEERVQEEAHCLVEELRKTNASPCDPTFILGAAPCNVICS
VIFQNRFDYTDQDFLSLMGKFNENFKILNSPWVQFCNCFPILFDYFPGSHRKAVKNIFYV
KNYITEQ/IKEHQK
FUNCTION Enzyme activity [-] (compared to cytochrome P450 2C2)
///
Îã2
CROSS-REFERENCE SYISC3
PROTEIN Chalcone synthase (CHS); naringenin-chalcone synthase
SOURCE Sinapis alba
N-TERMINAL MVMGT
CROSS-REFERENCE SYNPHS
PROTEIN Stilbene synthase (STS); trihydroxystilbene synthase
SOURCE Arachis hypogaea
N-TERMINAL MVSVS
EXPRESSION-SYSTEM Escherichia coli
CHANGE-CHIMERA (Met--Val 1-107 of CHS) - (Pro--Ile 103-389 of STS)
-POINT Pro 42 Ala (numbered as in CHS)
-SEQUENCE MVMGTPSSLDEIRKAQRADGPAGILAIGTANPANHVIQAEY(A)DYYFRITNSEHMTDLK
EKKRMCDKSTIRKRHMHLTEEFLKDNPNMCAYMAPSLDARQDIVVVEV/PRVGKEAATKA
IKEWGQPMSKITHLIFCTTSGVALPGVDYELIVLLGLDPSVKRYMMYHQGCFAGGTVLRL
AKDLAENNKDARVLIVCSENTAVTFRGPSETDMDSLVGQALFADGAAAIIIGSDPVPEVE
NPLFEIVSTDQKLVPNSHGAIGGLLREVGLTFYLNKSVPDIISQNINDALSKAFDPLGIS
DYNSIFWIAHPGGPAILDQVEQKVNLKPEKMNATRDVLSNYGNMSSACVFFIMDLMRKKS
LEEGLKTTGEGLDWGVLFGFGPGLTIETVVLRSVAI
FUNCTION CHS activity [0] (compared to CHS): STS activity [0] (compared
to STS)